Sains Malaysiana 53(2)(2024): 249-265

http://doi.org/10.17576/jsm-2024-5302-02

Progeny Evaluation from the Crossing of Novelty-Type Phalaenopsis I Hsin Bee x Phalaenopsis pulcherrima var. champorensis

(Penilaian Progeni daripada Kacukan Jenis Kebaharuan Phalaenopsis I Hsin Bee x Phalaenopsis pulcherrima var. champorensis)

 

DEDEH SITI BADRIAH, DEWI PRAMANIK, SUSKANDARI KARTIKANINGRUM, MINANGSARI DEWANTI, MAWADDAH, SURYAWATI, EKA FIBRIANTY, AGUS MUHARAM* & KURNIAWAN BUDIARTO

 

Research Center for Horticulture and Estate Crops, National Agency for Research and Innovation, Cibinong Science Center, Jl. Raya Jakarta Bogor, Cibinong, Bogor 16915, Indonesia

 

Diserahkan: 6 Jun 2023/Diterima: 22 Januari2024

 

Abstracts

The floriculture industry has rapidly developed in Indonesia. One of the potential genera of orchids is Phalaenopsis. New varieties must be developed to compete with imported ones. Genetic improvement of Phalaenopsis has been carried out through interspecific and intergeneric crossing - the crossing between P. I Hsin Bee x P. pulcherrima var. champorensis is dedicated to introduce harlequin type to P. pulcherrima. Forty progenies of the first generation (F1) and their parents are  used for the characterization and evaluation. The results show that P. pulcherrima is more dominant (50%) in flower shape and color based on the progeny composition. The introduction of the harlequin type into P. pulcherrima is indicated by 35% of progenies have  dark color at the flower and matched with the spotted pattern of the female parent, indicating that the female contributed partially to the respected traits. The progenies are divided into five distinct groups based on nine qualitative characteristics. Three progenies, namely D829-4, D829-6, and D829-8, are considered to have prominent features, such as more stable and symmetric coalescence of red-brownish blotches on the flowers, representing harlequin type than other progenies. These progenies will potentially be developed to be commercial varieties.

 

Keywords: Harlequin; hybridization; moon orchid; qualitative characters; quantitative characters

 

Abstrak

Industri florikultur telah berkembang pesat di Indonesia. Salah satu genus orkid yang berpotesi ialahPhalaenopsis. Varieti baharu daripada genus tersebut harus dikembangkan untuk menandingi varieti import. Penambahbaikan genetik Phalaenopsis telah dilakukan melalui kacukan antara spesies dan antara genus. Kacukan antara P. I Hsin x P. pulcherrima var. champorensis dilakukan untuk mengintegrasikan ciri harlequin pada P. pulcherrima. Sebanyak 40 progeni generasi pertama (F1) dan induk mereka telah digunakan untuk penilaian dan pencirian. Hasil menunjukkan bahwa P. pulcherrima lebih dominan (50%) dalam ciri bentuk dan warna bunga berdasarkan komposisi progeni. Integrasi ciriharlequin ke dalam P. pulcherrima ditunjukkan dengan banyaknya progeni (35%) yang mempunyai bunga berwarna hitam yang sama dengan pola bercak pada induk betina. Sembilan ciri kualitatif yang membahagikan kesemuaprogeni menjadi lima kumpulan, berdasarkan variasi warna dan bentuk daripada petal, sepal dorsal dan lateral, serta lobus-tengah. Tiga progeni, iaitu D829-4, D829-6 dan D829-8, menunjukkan ciri yang menonjol, seperti lebih stabil, gabungan simetri tompok merah kecoklatan pada bunga yang memperlihatkan ciri harlequin berbanding dengan progeni lain. Ketiga-tiga progeni tersebut memiliki potensi untuk dikembangkan lebih lanjut menjadi varieti komersial.

 

Kata kunci: Ciri kualitatif; cirikuantitatif; harlequin; kacukan; orkid bulan

RUJUKAN

Bolaños-Villegas, P. & Chen, F.C. 2022. Advances and perspectives for polyploidy breeding in orchids. Plants 11(11): 1-18. https://doi.org/10.3390/plants11111421

Cai, J., Liu, X., Vanneste, K., Prost, S., Tsai, W.C., Liu, K.W., Chen, L.J., He, Y., Xu, Q., Bian, C., Zheng, Z., Sun, F., Liu, W., Hsiao, Y., Pan, Z.J., Hsu, C.C., Yang, Y.P., Hsu, Y.C., Chuang, Y.C. & Liu, Z.J. 2015. The genome sequence of the orchid Phalaenopsis equestris. Nature Genetics 47(1): 65-72. https://doi.org/10.1038/ng.3149

Chen, C. 2020. The fundamental issue in the phalaenopsis industry in the Netherlands. Journal of Agriculture and Forestry 67(2): 89-100.

Chen, T.C., Lin, Y.Y. & Chen, W.H. 2020. Breeding of Harlequin flowers in Phalaenopsis orchid. In Orchid Biotechnology IV, edited by Chen, W.H. & Chen, H.H. World Scientific. pp. 15-41. https://doi.org/ doi:10.1142/9789811217777_0002

De, L. 2020. Morphological diversity in orchids. International Journal of Botany Studies International 5(5): 299-238. https://doi.org/10.13140/RG.2.2.24041.31849

Dwiati, M., Susanto, A.H. & Prayoga, L. 2020. Intergeneric hybrids of Phalaenopsis 2166 x Vanda ‘Saint Valentine’ showing maternal inheritance: A genetic analysis based on the partial gene. Biodiversitas 21(11): 5138-5145. https://doi.org/10.13057/biodiv/d211119

Hartati, S. 2010. The intergeneric crossing of Phalaenopsis sp. and Vanda tricolor. Journal of Biotechnology and Biodiversity 1(1): 32-36.

Hsiao, Y.Y., Pan, Z.J., Hsu, C.C., Yang, Y.P., Hsu, Y.C., Chuang, Y.C., Shih, H.H., Chen, W.H., Tsai, W.C. & Chen, H.H. 2011. Research on orchid biology and biotechnology. Plant and Cell Physiology 52(9): 1467-1486. https://doi.org/10.1093/pcp/pcr100

Hsu, C.C., Chen, H.H. & Chen, W.H. 2018. Phalaenopsis. In Ornamental Crops, Handbook of Plant Breeding 11, edited by van Hulenbroeck, J. Berlin Heidelberg: Springer pp. 573-625. https://doi.org/10.1007/978-3-319-90698-0

Hsu, C.C., Su, C.J., Jeng, M.F., Chen, W.H. & Chen, H.H. 2019. A HORT1 retrotransposon insertion in the PeMYB11 promoter causes harlequin/black flowers in Phalaenopsisorchids. Plant Physiology 180(3): 1535-1548. https://doi.org/10.1104/pp.19.00205

Hsu, S.C., Cheng, T.C., Bolaños-Villegas, P., Chin, S. W., & Chen, F. C. (2010). Pollen meiotic behavior about phalaenopsis breeding. Acta Horticulturae 878: 139-144. https://doi.org/10.17660/actahortic.2010.878.15

Hu, X., Lan, S., Song, X., Yang, F., Zhang, Z., Peng, D. & Ren, M. 2021. Genetic divergence between two sympatric ecotypes of Phalaenopsis pulcherrima on Hainan Island. Diversity 13(9): 1-17. https://doi.org/10.3390/d13090446

Huang, J-Z., Lin, C-P., Cheng, T-C., Chang, B.C-H., Cheng, S-Y., Chen, Y-W., Lee, C-Y., Chin, S-W. & Chen, F-C. 2015. A de novo floral transcriptome reveals clues into Phalaenopsis orchid flower development. PLoS ONE 10(5): e0123474. https://doi.org/10.1371/journal.pone.0123474

Kassambara, A. & Mundt, F. 2020. Factoextra: Extract and visualize the results of multivariate data analyses. CRAN.R-project.org.

Lee, Y.I. & Chung, M.C. 2020. Chromosome analysis of Phalaenopsis Harlequin-type cultivars. In Orchid Biotechnology IV. World Scientific. pp. 87-94. https://doi.org/ doi:10.1142/9789811217777_0005

Li, C., Dong, N., Zhao, Y., Wu, S., Liu, Z. & Zhai, J. 2021. A review for the breeding of orchids: Current achievements and prospects. Horticultural Plant Journal 7(5): 380-392. https://doi.org/10.1016/j.hpj.2021.02.006

Liang, C.Y., Rengasamy, K.P., Huang, L.M., Hsu, C.C., Jeng, M.F., Chen, W.H. & Chen, H.H. 2020. Assessment of violet-blue color formation in Phalaenopsis orchids. BMC Plant Biology 20(1): 1-16. https://doi.org/10.1186/s12870-020-02402-7

Lin, S., Lee, H.C., Chen, W.H., Chen, C.C., Kao, Y.Y., Fu, Y.M., Chen, Y.H. & Lin, T.Y. 2001. Nuclear DNA contents of Phalaenopsis sp. and Doritis pulcherrima. Journal of the American Society for Horticultural Science 126(2): 195-199. https://doi.org/ 10.21273/jashs.126.2.195

Liu, W.L., Shih, H.C., Weng, I.S., Ko, Y.Z., Tsai, C.C., Chou, C.H. & Chiang, Y.C. 2016. Characterization of genomic inheritance of intergeneric hybrids between Ascocenda and Phalaenopsis cultivars by GISH, PCR-RFLP, and RFLP. PLoS ONE 11(4): 1-14. https://doi.org/10.1371/journal.pone.0153512

Marwoto, B., Badriah, D.S., Dewanti, M. & Sanjaya, L. 2012. Persilangan interspesifik dan intergenerik anggrek Phalaenopsis untuk menghasilkan hibrid tipe baru. Prosiding Seminar Nasional Anggrek, Makalah Pendukung 3: 101-116.

Meyer, N. 2010. Comparaison de variantes de régressions logistiques PLS et de régression PLS sur variables qualitative: Application aux données d’allélotypage [Comparing the linear and the logistic PLS regression with qualitative predictors: Application to allelotyping data] (M. Maumy-Bertrand & F. Bertrand, Trans.). Journal de La Société Française de Statistique 151(2): 1-18.

Nurmalinda, Kartikaningrum, S., Hayati, N.Q. & Widyastoety, D. 2011. Preferensi konsumen terhadap anggrek Phalaenopsis, Vanda, dan Dendrobium. Jurnal Hortikultura 21(4): 372. https://doi.org/10.21082/jhort.v21n4.2011.p372-384

Pramanik, D., Spaans, M., Kranenburg, T., Bogarin, D., Heijungs, R., Lens, F., Smets, E., & Gravendeel, B. 2022. Inflorescence lignification of natural species and horticultural hybrids of Phalaenopsis orchids. Scientiae Horticulturae 295:110845. https://doi.org/10.1016/j.scientia.2021.110845

R Team. 2019. RStudio: Integrated Development for R. R Foundation for Statistical Computing.

R Team. 2016. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing.

Schrader, J., Shi, P., Royer, D.L., Peppe, D.J., Gallagher, R.V., Li, Y., Wang, R. & Wright, I.J. 2021. Leaf size estimation based on leaf length, width and shape. Annals of Botany 128(4): 395-406. https://doi.org/10.1093/aob/mcab078

Tang, C.Y. & Chen, W.H. 2007. Breeding and development of new varieties in Phalaenopsis. In Orchid Biotechnology, edited by Chen, W.H. & Chen, H.H. pp. 1-22. World Scientific Publishing Co. Ltd.

Tsai, C.C., Chiang, Y.C., Huang, S.C., Liu, W.L. & Chou, C.H. 2010. Intergeneric hybridization, embryo rescue, and molecular detection for intergeneric hybrids between Ascocenda and Phalaenopsis. Acta Horticulturae 829: 413-416. https://doi.org/10.17660/actahortic.2009.829.67

van Tongerlo, E., van Ieperen, W., Dieleman, J.A. & Marcelis, L.F.M. 2021. Vegetative traits can predict flowering quality in Phalaenopsis orchids despite large genotypic variation in response to light and temperature. PLoS ONE 16: 1-22. https://doi.org/10.1371/journal.pone.0251405

Vo, T.C., Seo, J.W., Kim, C.K., Kim, H.Y. & Lim, K.B. 2019. Breeding of the mini-type phalaenopsis cultivar ‘Yellow Green’, having deep-yellow flowers with a red lip. Horticultural Science and Technology 37(4): 540-547. https://doi.org/10.7235/ HORT.20190054

Watanabe, K.N. & Watanabe, J.A. 2000. Genetic diversity and molecular genetics of ornamental plant species. Biotechnology & Biotechnological Equipment 14(2): 19-21. https://doi.org/10.1080/13102818.2000.10819081

Yuan, S.C., Chin, S.W. & Chen, F.C. 2015. Current trends of Phalaenopsis orchid breeding and study on pollen storage. Acta Horticulturae 1078: 19-23. https://doi.org/ 10.17660/ActaHortic.2015.1078.1

Yukawa, T., Kita, K., Handa, T., Hidayat, T. & Ito, M. 2005. Molecular phylogenetics of Phalaenopsis (Orchidaceae) and allied genera: Re-evaluation of generic concepts. Acta Phytotaxonomica et Geobotanica 56(2): 141-161.

Zhang, D., Zhao, X-W., Li, Y-Y., Ke, S-J., Yin, W-L., Lan, S. & Liu, Z-J. 2022. Advances and prospects of orchid research and industrialization. Horticulture Research 9: uhac220. https://doi.org/10.1093/hr/uhac220

Zhang, Z., Gale, S.W., Li, J.H., Fischer, G.A., Ren, M.X. & Song, X.Q. 2019. Pollen-mediated gene flow ensures connectivity among spatially discrete sub-populations of Phalaenopsis pulcherrima, a tropical food-deceptive orchid. BMC Plant Biology 19(1): 1-16. https://doi.org/10.1186/s12870-019-2179-y

 

*Pengarang untuk surat-menyurat; email: agus.muharam55@gmail.com

 

 

 

 

 

 

 

   

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